OpenCRISPR-1 mRNA for gene editing
OpenCRISPR-1 mRNA encodes a computationally designed CRISPR-Cas genome editor developed through large-scale protein language modeling of natural CRISPR-Cas sequences. The mRNA is optimized for mammalian expression and encodes a highly functional genome editor with enhanced specificity and reduced off-target effects compared to natural Cas proteins. The OpenCRISPR-1 system demonstrates superior editing efficiency across multiple target sites and cell types, making it suitable for research applications and potential therapeutic development.
Note: The sequence of OpenCRISPR ORF is licensed to Cisterna by Profluent Bio Inc. for research applications. Prohibited uses include human germline modifications, gene drives, and sterile seed technology. Customers pursuing commercial development should contact legal@profluent.bio regarding licensing.
1) Ruffolo, J. A., Nayfach, S., Gallagher, J., Bhatnagar, A., Beazer, J., Hussain, R., Russ, J., Yip, J., Hill, E., Pacesa, M., Meeske, A. J., Cameron, P., & Madani, A. (2025). Design of highly functional genome editors by modelling CRISPR-Cas sequences. Nature, 645(8080), 518–525
2) Ruffolo, J. A., Nayfach, S., Gallagher, J., Bhatnagar, A., Beazer, J., Hussain, R., Russ, J., Yip, J., Hill, E., Pacesa, M., Meeske, A. J., Cameron, P., & Madani, A. (2024). Design of highly functional genome editors by modelling the universe of CRISPR-Cas sequences
3) https://github.com/Profluent-AI/OpenCRISPR/blob/main/OpenCRISPR-1/OpenCRISPR-1.fasta
Cisterna's off-the-shelf RNAs are validated and ready to order through our quick quote system. Simply follow these steps: Step 1 (Find your RNA) If you already know what you need, select your desired RNA directly from the main page. If you'd like to learn more first, browse by category, click on the RNA name to view its product page, and explore usage examples and references before ordering. Step 2 (Add to Cart) Choose your preferred 'Quality', 'Amount', and 'Quantity', then click 'Add to Cart'. Repeat for any additional items. Step 3 (Get a Quote) When all items are in your cart, click 'Get a Quote'. Fill in your name, professional email, and academic or business address. Please note that personal email addresses and home addresses will not be accepted. Step 4 (Save your Quote) Once your information is complete, you can download your quote as a PDF for your records. Step 5 (Checkout) Click 'Continue to Checkout' to proceed to the final order page. If your institution requires it, you can enter an optional Purchase Order (PO) number here. Review your order and click 'Place an Order'. That's it! We'll take it from here. Your RNA will be synthesized and shipped as quickly as possible. Invoices are sent electronically upon confirmation of delivery, and all invoices are issued on Net30 terms.
PRIME is Cisterna's core RNA production platform, capable of synthesizing all RNA types including mRNA, tRNA, siRNA, sgRNA, aptamers, and more. The PRIME platform is engineered to drastically reduce product-related impurities such as dsRNA, short-truncated products, abortive transcripts, and DNA:RNA hybrids, all commonly generated in standard in vitro transcription. The result is consistently high-quality RNAs that are fully functional and well-suited for most downstream applications such as X-ray crystallography, Cryo-EM, tRNA aminoacylation and protein expression.
Both PRIME and SPECC reflect Cisterna’s commitment to producing high-quality RNA, but they are designed to serve different needs. PRIME is Cisterna’s standard production platform and is applicable to all RNA types. It delivers high-purity RNA suitable for a wide range of applications, from structural and biochemical studies to robust protein expression. For most research applications, PRIME offers excellent performance and exceptional value at an affordable price point. SPECC (SPEcific Capture and Cleavage) is Cisterna’s flagship purification system and is currently available for mRNA only. It takes purity a step further by selectively isolating full-length mRNA transcripts, enabling near-HPLC-level purity. SPECC is the ideal choice when the highest possible mRNA quality is desired, such as in rigorous quantitative studies, high-dose applications, or therapeutic development.