Name: Mango-3 WT FL Aptamer
Brand: Cisterna Biologics
Price: $ 132.00 USD USD
Availability: InStock

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‍Mango-3 WT FL Aptamer is a compact 45-nucleotide fluorogenic RNA aptamer that binds the thiazole orange derivative TO1-Biotin with high, low-nanomolar affinity and switches on its fluorescence by more than 1,000-fold, producing a bright green signal (emission ~535 nm) with an exceptionally high fluorescence quantum yield (~0.55). Fluorescence activation occurs because the aptamer folds into a compact G-quadruplex that, together with an embedded non-canonical duplex, clamps the otherwise non-fluorescent dye into a rigid, planar conformation. This makes Mango-III one of the brightest light-up aptamers and a powerful tool for visualizing RNA directly in biological systems without the need for protein-based tags.
Unlike traditional fluorescent reporter systems that rely on bulky protein fusions, Mango-3 is a small, genetically encodable RNA tag that can be expressed inside living cells, enabling real-time tracking of RNA molecules — including short, structured non-coding RNAs — with minimal structural disruption. Its high brightness and tight dye binding also make it especially well suited to single-molecule and array-based imaging.

Common research applications include:

Live-cell RNA imaging: Tagging mRNAs, lncRNAs, or small non-coding RNAs to track their localization, movement, and dynamics within living cells in real time
Single-molecule RNA tracking: Using tandem Mango arrays to image and follow individual RNA molecules with high signal-to-noise
RNA transcription monitoring: Fusing Mango-3 to an RNA of interest to monitor transcription levels and kinetics by fluorescence microscopy or plate-based assays
RNA secondary structure studies: Using Mango-III's well-characterized structure (PDB: 6E8S) to investigate RNA folding and aptamer–ligand interactions
Biosensor development: Incorporating Mango-3 into RNA-based biosensor platforms to detect specific metabolites, proteins, or nucleic acid targets
RNA pull-down and affinity purification: Exploiting the high-affinity TO1-Biotin interaction to capture and isolate tagged RNAs and their binding partners

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If you desire custom RNA fused with Mango-3 WT FL Aptamer, please use the custom RNA tab and fill in the details to get a custom quote.

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1) Dolgosheina EV, et al. RNA Mango aptamer-fluorophore: a bright, high-affinity complex for RNA labeling and tracking. ACS Chem Biol. 2014;9(10):2412–2420.
2) Trachman RJ, et al. Structure and functional reselection of the Mango-III fluorogenic RNA aptamer. Nat Chem Biol. 2019;15(5):472–479.
3) Autour A, et al. Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells. Nat Commun. 2018;9:656.
4) Cawte AD, Unrau PJ, Rueda DS. Live cell imaging of single RNA molecules with fluorogenic Mango II arrays. Nat Commun. 2020;11:1283.
5) Jeng SCY, et al. Fluorophore ligand binding and complex stabilization of the RNA Mango and RNA Spinach aptamers. RNA. 2016;22(12):1884–1892.

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FAQs

How do I purchase Off-the-shelf RNAs?

Cisterna's off-the-shelf RNAs are validated and ready to order through our quick quote system. Simply follow these steps: Step 1 (Find your RNA) If you already know what you need, select your desired RNA directly from the main page. If you'd like to learn more first, browse by category, click on the RNA name to view its product page, and explore usage examples and references before ordering. Step 2 (Add to Cart) Choose your preferred 'Quality', 'Amount', and 'Quantity', then click 'Add to Cart'. Repeat for any additional items. Step 3 (Get a Quote) When all items are in your cart, click 'Get a Quote'. Fill in your name, professional email, and academic or business address. Please note that personal email addresses and home addresses will not be accepted. Step 4 (Save your Quote) Once your information is complete, you can download your quote as a PDF for your records. Step 5 (Checkout) Click 'Continue to Checkout' to proceed to the final order page. If your institution requires it, you can enter an optional Purchase Order (PO) number here. Review your order and click 'Place an Order'. That's it! We'll take it from here. Your RNA will be synthesized and shipped as quickly as possible. Invoices are sent electronically upon confirmation of delivery, and all invoices are issued on Net30 terms.

What is PRIME quality?

PRIME is Cisterna's core RNA production platform, capable of synthesizing all RNA types including mRNA, tRNA, siRNA, sgRNA, aptamers, and more. The PRIME platform is engineered to drastically reduce product-related impurities such as dsRNA, short-truncated products, abortive transcripts, and DNA:RNA hybrids, all commonly generated in standard in vitro transcription. The result is consistently high-quality RNAs that are fully functional and well-suited for most downstream applications such as X-ray crystallography, Cryo-EM, tRNA aminoacylation and protein expression.

What is the difference between PRIME quality versus SPECC quality RNA?

Both PRIME and SPECC reflect Cisterna’s commitment to producing high-quality RNA, but they are designed to serve different needs. PRIME is Cisterna’s standard production platform and is applicable to all RNA types. It delivers high-purity RNA suitable for a wide range of applications, from structural and biochemical studies to robust protein expression. For most research applications, PRIME offers excellent performance and exceptional value at an affordable price point. SPECC (SPEcific Capture and Cleavage) is Cisterna’s flagship purification system and is currently available for mRNA only. It takes purity a step further by selectively isolating full-length mRNA transcripts, enabling near-HPLC-level purity. SPECC is the ideal choice when the highest possible mRNA quality is desired, such as in rigorous quantitative studies, high-dose applications, or therapeutic development.

Mango-3 WT FL Aptamer

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