E coli elongator tRNA coding for Methionine CAT anti-codon
E. coli tRNA-Met (CAT), elongator is a 77-nucleotide transfer RNA that delivers methionine to the ribosomal A site during chain elongation. E. coli carries two functionally distinct classes of methionine tRNA: the initiator tRNA-fMet, which is formylated and used to start translation, and the elongator tRNA-Met supplied here, which is never formylated and functions only in elongation. Although both share the CAU anticodon, they are distinguished at the top of the acceptor stem, the initiator carries a C1·A72 mismatch required for recognition by methionyl-tRNA formyltransferase, whereas the elongator has a standard Watson-Crick base pair and is therefore excluded from initiation and handed instead to EF-Tu. This product is the wild-type sequence encoded at the metU locus (identical to metT), supplied as an unmodified in vitro transcript with an intact 3′ CCA end, ready for aminoacylation by methionyl-tRNA synthetase or by flexizyme.
Applications:
1) EcoCyc ID: EG30059 (metU; b0666; ECK0657)
2) Varshney, U., Lee, C.P., & RajBhandary, U.L. (1993). From elongator tRNA to initiator tRNA. Proceedings of the National Academy of Sciences, 90(6), 2305-2309
3) Guillon, J.M., Mechulam, Y., Blanquet, S., & Fayat, G. (1993). Importance of formylability and anticodon stem sequence to give a tRNA(Met) an initiator identity in Escherichia coli. Journal of Bacteriology, 175(14), 4507-4514
Cisterna's off-the-shelf RNAs are validated and ready to order through our quick quote system. Simply follow these steps: Step 1 (Find your RNA) If you already know what you need, select your desired RNA directly from the main page. If you'd like to learn more first, browse by category, click on the RNA name to view its product page, and explore usage examples and references before ordering. Step 2 (Add to Cart) Choose your preferred 'Quality', 'Amount', and 'Quantity', then click 'Add to Cart'. Repeat for any additional items. Step 3 (Get a Quote) When all items are in your cart, click 'Get a Quote'. Fill in your name, professional email, and academic or business address. Please note that personal email addresses and home addresses will not be accepted. Step 4 (Save your Quote) Once your information is complete, you can download your quote as a PDF for your records. Step 5 (Checkout) Click 'Continue to Checkout' to proceed to the final order page. If your institution requires it, you can enter an optional Purchase Order (PO) number here. Review your order and click 'Place an Order'. That's it! We'll take it from here. Your RNA will be synthesized and shipped as quickly as possible. Invoices are sent electronically upon confirmation of delivery, and all invoices are issued on Net30 terms.
PRIME is Cisterna's core RNA production platform, capable of synthesizing all RNA types including mRNA, tRNA, siRNA, sgRNA, aptamers, and more. The PRIME platform is engineered to drastically reduce product-related impurities such as dsRNA, short-truncated products, abortive transcripts, and DNA:RNA hybrids, all commonly generated in standard in vitro transcription. The result is consistently high-quality RNAs that are fully functional and well-suited for most downstream applications such as X-ray crystallography, Cryo-EM, tRNA aminoacylation and protein expression.
Both PRIME and SPECC reflect Cisterna’s commitment to producing high-quality RNA, but they are designed to serve different needs. PRIME is Cisterna’s standard production platform and is applicable to all RNA types. It delivers high-purity RNA suitable for a wide range of applications, from structural and biochemical studies to robust protein expression. For most research applications, PRIME offers excellent performance and exceptional value at an affordable price point. SPECC (SPEcific Capture and Cleavage) is Cisterna’s flagship purification system and is currently available for mRNA only. It takes purity a step further by selectively isolating full-length mRNA transcripts, enabling near-HPLC-level purity. SPECC is the ideal choice when the highest possible mRNA quality is desired, such as in rigorous quantitative studies, high-dose applications, or therapeutic development.