E coli tRNA coding for Leucine CAG anti-codon
E. coli tRNA^Leu(CAG) is an 87-nucleotide transfer RNA that decodes CUG leucine codons via its CAG anticodon and is aminoacylated by leucyl-tRNA synthetase (LeuRS). It adopts the canonical cloverleaf secondary structure and L-shaped tertiary fold characteristic of all tRNAs, with a long variable arm, a hallmark of leucine and serine tRNAs, that serves as a key identity element for LeuRS recognition. The anticodon loop, acceptor stem, and variable arm have been extensively characterized in the context of codon–anticodon interactions, wobble decoding, aminoacyl-tRNA synthetase specificity, and suppressor tRNA engineering. It has broad utility as a model tRNA for in vitro translation and as a scaffold for synthetic biology and orthogonal translation system development.
Applications -
1) RNAcentral ID: URS000044A612_511145
2) Soma, A., et al. (1996). "Anticodon loop mutations of Escherichia coli tRNA^Leu." Nucleic Acids Research, 24(11), 2127–2134
3) Soma, A. & Himeno, H. (1998). "Cross-species aminoacylation of tRNA with a long variable arm between Escherichia coli leucyl- and seryl-tRNA synthetases." Nucleic Acids Research, 26(20), 4743–4748
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