Chili FL Aptamer is a fluorescent RNA aptamer consisting of 52 nucleotides that activates fluorescence through a unique mechanism of intermolecular proton transfer to guanine, producing a large Stokes shift. This structural property allows Chili to bind and activate specific fluorogenic dyes, generating a bright, detectable fluorescent signal upon binding. This makes it a powerful tool for visualizing RNA directly in biological systems without the need for protein-based tags. Unlike traditional fluorescent reporter systems that rely on bulky protein fusions, Chili is a compact RNA-based tag that can be genetically encoded and expressed inside living cells, enabling real-time tracking of RNA molecules with minimal structural disruption.
Common research applications include:
If you desire custom RNA fused with Chili FL Aptamer, please use the custom RNA tab and fill in the details to get a custom quote.
Cisterna's Off-the-shelf are validated to be purchased with a quick quote generated by the system. This means you go through a series of quick steps as below to complete your purchase. Step 1: If you already know the RNA you want, select the RNA you desire on the main page by clicking the 'Quality', 'Amount', and 'Quantity' on the main page and clicking on 'Add to Cart' button. If you want to read more about the individual RNA, click on the category of your RNA and select the RNA you desire by directly clicking on its name. This will take you to the individual RNA page, where you can learn more about the RNA, its usage in research with top examples for each of the use cases under the 'References' tab. Step 2: Once you are ready to order, just select the 'Quality', 'Amount', and 'Quantity', then click on 'Add to Cart' button. Step 3: After all the items have been added to the Cart, Click on 'Get a quote' button. Step 4: Fill in all of the information such as name, your professional email, and academic or business address. Note that non-professional emails and home addresses will not be considered. Step 5: Once the information is filled in, you may download the quote as PDF and save it for your reference. Step 6: You can then click on 'Continue to checkout' button which will take you to the final 'Checkout' page. Step 7: You can provide an optional Purchase Order (PO) number here as some institutions may require this. If everything looks ok, then click on 'Place an order'. Thats it! You are done. We will take it from here. We will make your RNA and ship in the fastest possible time. Invoices will be sent electronically to the email after confirmation of delivery. All invoices have a Net30 agreement.
PRIME is the standard quality RNA made by Cisterna. All RNAs including mRNAs are made with PRIME quality. The PRIME platform generates very low impurities such as dsRNA, short-truncated products, abortive transcripts and DNA:RNA hybrids. The result is a highly pure RNA that is functional and can be used for most downstream applications demanding high-purity such as X-ray crystallography, Cryo-EM, tRNA aminoacylation and to generate maximum amount of functional proteins.
Both PRIME and SPECC reflect Cisterna's commitment to high-quality RNA, but they are designed for different needs and applications. PRIME is Cisterna's standard production system and is applicable to all RNA types. It delivers very high purity RNA suitable for a wide range of demanding applications, from structural studies to functional protein expression. SPECC (SPEcific Capture and Cleavage) is Cisterna's flagship system and is currently applicable to mRNA only. It takes purity a step further by selectively isolating full-length mRNA transcripts, approaching HPLC-level purity. SPECC is the ideal choice when the highest possible mRNA quality is required, particularly for quantitative studies, high-dose applications, and therapeutic development. In terms of cost, SPECC is approximately 5-10x the price of PRIME, reflecting the advanced purification processes involved. While affordably priced, PRIME still delivers very high RNA quality, well above what you might expect at this price point.